iPS向肝臟細(xì)胞定向分化試劑盒

• 型   號：Cellartis Y30055
• 價   格：13438

完整即用的iPS Cell to Hepatocyte Differentiation System是一套DIY系統(tǒng)，操作簡便，包含從起始的iPS細(xì)胞培養(yǎng)、iPS細(xì)胞向定型內(nèi)胚層（Definitive Endoderm，DE）細(xì)胞分化、DE細(xì)胞向肝臟細(xì)胞分化的全套產(chǎn)品，并且均為即用型，獲得的肝臟細(xì)胞純度高達(dá)90%，無需篩選純化，可以穩(wěn)定表達(dá)藥物代謝相關(guān)酶系統(tǒng)和藥物轉(zhuǎn)運系統(tǒng)；保留供體細(xì)胞遺傳背景。

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 華雅旗下紅榮微再授權(quán)代理（上海）TAKARA CellArtis，Rubicon產(chǎn)品  Cellartis-Y30055現(xiàn)貨數(shù)量有限 詳詢 1500 1904 520 紅榮微再-客服: 經(jīng)銷商專員

iPS向肝臟細(xì)胞定向分化試劑盒

產(chǎn)品英文名稱：Cellartis® iPS Cell to Hepatocyte Differentiation System

產(chǎn)品中文名稱：iPS向肝臟細(xì)胞定向分化試劑盒

iPS向肝臟細(xì)胞定向分化各個步驟的培養(yǎng)

產(chǎn)品介紹

以肝臟細(xì)胞為模型的實驗越來越受到重視，在肝臟病變機理研究、藥物代謝研究、藥物毒理評估等方面有廣闊的應(yīng)用前景。然而，以個體原代肝細(xì)胞為材料來源會產(chǎn)生如取材困難、材料批間差大、無法長期穩(wěn)定供應(yīng)等一系列難以克服的問題。因此，通過iPS批量轉(zhuǎn)化生產(chǎn)肝細(xì)胞的方法就顯示出特別的優(yōu)勢。

Cellartis iPS Cell to Hepatocyte Differentiation System可以穩(wěn)定地、規(guī)?；貙崿F(xiàn)iPS向hepatocyte的誘導(dǎo)轉(zhuǎn)化生產(chǎn)，而且誘導(dǎo)轉(zhuǎn)化出的肝細(xì)胞能夠穩(wěn)定表達(dá)藥物代謝相關(guān)酶系統(tǒng)和藥物轉(zhuǎn)運系統(tǒng)。

Cellartis iPS Cell to Hepatocyte Differentiation System是一套Do-It-Yourself系統(tǒng)，客戶既可以對某個特殊病人來源的iPS實現(xiàn)轉(zhuǎn)化，也可以利用cellartis供應(yīng)的iPS進(jìn)行標(biāo)準(zhǔn)化操作。使用本產(chǎn)品的過程起始于誘導(dǎo)人iPS轉(zhuǎn)化成為確定性內(nèi)胚層（Definitive Endoderm (DE)）細(xì)胞，然后誘導(dǎo)轉(zhuǎn)化DE至肝細(xì)胞。本產(chǎn)品是All-In-One型產(chǎn)品，實驗所需的培養(yǎng)基、基質(zhì)（coating）等包括在內(nèi)。應(yīng)用本產(chǎn)品轉(zhuǎn)化約3 × 10⁶ hiPS cells,可以獲得約5 × 10⁶ 肝細(xì)胞（大約50 cm²面積的單層貼壁細(xì)胞）。

利用Cellartis iPS Cell to Hepatocyte Differentiation System轉(zhuǎn)化得到的肝細(xì)胞除了清晰表現(xiàn)出肝細(xì)胞的*特征外（如肝細(xì)胞特異性標(biāo)志物、CYP系列酶等），還保留了原始細(xì)胞供體者的遺傳基因背景。另外，相比較從組織切除獲得的原代肝臟細(xì)胞（通常需要低溫保存），通過本產(chǎn)品系統(tǒng)轉(zhuǎn)化獲得的肝細(xì)胞在更長的時間跨度上保持著生理功能的穩(wěn)定性。這一點對于毒理學(xué)評估和病毒感染評估具有顯著的意義。

產(chǎn)品特點:

高度可重復(fù)的強大系統(tǒng) - *相同的方法已被證明可用于25種不同的iPS細(xì)胞系，因此無需針對您的細(xì)胞進(jìn)行優(yōu)化。

理想的藥物代謝和安全性研究 - 始終如一地生成具有不同遺傳背景的> 90％純的功能性hiPS細(xì)胞來源的肝細(xì)胞。

定制的起始材料 - 從任何疾病相關(guān)的iPS細(xì)胞系開始，創(chuàng)建準(zhǔn)確的肝臟疾病模型。

準(zhǔn)備好個性化醫(yī)療 - 為治療應(yīng)用制造針對患者的疾病特異性細(xì)胞。

擴展實驗窗口 - 肝細(xì)胞可用于功能測試至少11天。

Highly reproducible, robust system—the exact same protocol has been shown to work across 25 different iPS cell lines, so no need to optimize for your lines.

Ideal for drug metabolism and safety studies—consistently generate panels of >90% pure, functional, hiPS cell-derived hepatocytes with diverse genetic backgrounds.

Customized starting materials—start with any disease-relevant iPS cell lines and create accurate liver disease models.

Ready for personalized medicine—make patient-specific, disease-specific cells for therapeutic applications.

Extended experimental window—hepatocytes can be used for a minimum of 11 days for functional tests.

產(chǎn)品應(yīng)用:

iPS向肝臟細(xì)胞定向分化

組份：iPS細(xì)胞培養(yǎng)基+iPS分化內(nèi)胚層培養(yǎng)基+內(nèi)胚層分化肝細(xì)胞培養(yǎng)基

每種培養(yǎng)基都是*培養(yǎng)基

Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055)：

Cellartis DEF-CS 100 Culture System (Cat. No. Y30020)

? 100 ml DEF-CS 100 Basal Medium

? 800 µl DEF-CS COAT-1 (for 100 ml)

? 300 µl DEF-CS GF-1 (for 100 ml)

? 100 µl DEF-CS GF-2 (for 100 ml)

? 40 µl DEF-CS GF-3 (for 100 ml)

Cellartis Definitive Endoderm Differentiation Kit (Cat. No. Y30030)

? 1 tube Definitive Endoderm Differentiation Coating (10 ml)

? 1 bottle Definitive Endoderm Differentiation Day 0 (18 ml)

? 1 bottle Definitive Endoderm Differentiation Day 1 (18 ml)

? 1 bottle Definitive Endoderm Differentiation Day 2 (18 ml)

? 1 bottle Definitive Endoderm Differentiation Day 3 (25 ml)

? 1 bottle Definitive Endoderm Differentiation Day 4 (47 ml)

? 1 bottle Definitive Endoderm Differentiation Day 6 (47 ml)

Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050)

? 1 bottle Hepatocyte Thawing and Seeding Medium (1) (25 ml)

? 1 bottle Hepatocyte Progenitor Medium (2) (50 ml)

? 2 bottles Hepatocyte Maturation Medium Base (3A) (24 ml)

? 1 tube Hepatocyte Maturation Medium Supplement (3B) (2.6 ml)

? 3 bottles Hepatocyte Maintenance Medium (4) (50 ml)

? 1 tube Hepatocyte Coating (7.5 ml)

Cellartis-Y30055 iPS Cell to Hepatocyte Differentiation System詳詢

1500 1904 520 紅榮微再-客服: 經(jīng)銷商專員

自備試劑：

人多能干細(xì)胞

胎牛血清（FBS）或血清替代品（KO-SR）

PBS Dulbecco's（含有Ca2 +和Mg2 +的D-PBS + / +）

PBS Dulbecco's不含Ca 2+和Mg 2+（D-PBS - / - ）

TrypLE選擇酶（1X），不含酚紅

保存：

iPS細(xì)胞培養(yǎng)基添加劑–20°C，基礎(chǔ)培養(yǎng)基和包被劑2–8°C

iPS分化內(nèi)胚層培養(yǎng)基和內(nèi)胚層分化肝細(xì)胞培養(yǎng)基所有組份都保存在–20°C。

Cellartis DEF-CS 100 Culture System (Y30020)：DEF-CS 100 Basal Medium ，COAT-1   2–8°C；GF-1, GF-2, and GF-3  –20°C

Cellartis Definitive Endoderm Differentiation Kit (Y30030)：–20°C

Cellartis Hepatocyte Differentiation Kit (Y30050) ：–20°C

產(chǎn)品性能：

Immunocytochemistry analysis of hepatocyte differentiation. hiPS cells were differentiated into functional hepatocytes using the Cellartis iPS Cell to Hepatocyte Differentiation System. Hepatocytes were immunostained to detect early hepatic markers HNF4α (red, nuclear) and CK18 (green) on days 14 and 21. As the hepatocytes matured, expression of liver-specific markers CYP3A (red, cytoplasmic) and Albumin (green) increased as seen on day 28, as expected. Cell nuclei were stained with DAPI (blue).

參考文獻(xiàn)：

Asplund, A., et al. (2015) Stem Cell Rev. and Rep. Advance online publication. doi: 10.1007/s12015-015-9621-9

訂購信息

肝細(xì)胞培養(yǎng)完整解決方案

人iPS誘導(dǎo)分化成肝細(xì)胞全過程的完整試劑盒：Y30055（新產(chǎn)品）

人iPS來源肝細(xì)胞株及*培養(yǎng)基Y10133/Y10134/Y10135（新產(chǎn)品）

人原代肝臟細(xì)胞培養(yǎng)基 Y20020（新產(chǎn)品）

http://www.chem17.com/st321032/article_1496885.html

華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司主營細(xì)胞治療和精準(zhǔn)醫(yī)療等產(chǎn)品，2018年與TAKARA繼續(xù)合作，授權(quán)代理（上海）其旗下Cellartis干細(xì)胞研究制品和Rubicon單細(xì)胞文庫構(gòu)建試劑。Cellartis公司位于瑞典，擁有iPS細(xì)胞等干細(xì)胞分化為肝細(xì)胞及臟器細(xì)胞的分化誘導(dǎo)技術(shù)和ES細(xì)胞，ips細(xì)胞，分化細(xì)胞等干細(xì)胞相關(guān)產(chǎn)品。

Cellartis-Y30055 iPS Cell to Hepatocyte Differentiation System 手機1500 1904 520 紅榮微再-客服  經(jīng)銷商專員