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多能細胞檢測用單克隆抗體
產品英文名稱:Monoclonal Antibodies for Detecting Pluripotent Cells
產品中文名稱:Cellect系列多能細胞檢測用單克隆抗體
用途:鑒別、分析、分離具有發(fā)育多能性的人類胚胎干細胞(ES)和誘導多能干細胞(iPS)。
hES-Cellect 識別人多能細胞
ES-Cellect 識別人和鼠多能細胞
hFF-Cellect 識別人成纖維細胞
Y20010 hES-Cellect™ 150 μg
Y20011 ES-Cellect™ 150 μg
Y20012 hFF-Cellect™ 150 μg
Cellect系列單克隆抗體用于鑒別、分析、分離具有發(fā)育多能性的人類胚胎干細胞(ES)和誘導多能干細胞(iPS)。本系列抗體特異性地識別具有發(fā)育多能性的干細胞表面的抗原,而這些抗原在干細胞定向分化后會被弱化調控。
產品特點
hES-Cellect和ES-Cellect是識別多能細胞特異性表面標記的高純度單克隆抗體
hFF-Cellect抗體識別人成纖維細胞,并具有比SSEA和TRA抗體更低的交叉反應性
這些抗體使用時不需要細胞固定或破膜來檢測和選擇活細胞
hES-Cellect and ES-Cellect are high-purity monoclonal antibodies that recognize surface markers specific to pluripotent cells
The hFF-Cellect antibody recognizes human fibroblasts and has lower cross reactivity than SSEA and TRA antibodies that recognize fibroblasts and other human somatic cells
These antibodies can be used without cell fixation or permeabilization to detect and select live cells
產品參數(shù)
成分:150 μg親和純化鼠單克隆抗體
同種型isotype:IgG1
免疫原:
產品名稱 | 免疫原immunogen |
hES-Cellect | 純化、未分化hES細胞 |
ES-Cellect | 純化、未分化hES細胞 |
hFF-Cellect | 純化hFF細胞 |
特異性:
hES-Cellect 識別人ES胚胎干細胞和iPS誘導多能干細胞,hFF、mEF和mES陰性
ES-Cellect 識別人ES胚胎干細胞和iPS誘導多能干細胞及鼠ES胚胎干細胞,hFF、mEF陰性
hFF-Cellect 識別人FF成纖維細胞,hES和hiPS陰性。
hES細胞:human embryonic stem,人胚胎干細胞; hFF細胞:human foreskin fibroblast (hFF),人皮膚成纖維細胞 。mEF細胞:mouse Embryonic Feeder 鼠胚胎飼養(yǎng)細胞;mES細胞: mouse Embryonic Stem 鼠胚胎干細胞
Product Name | Human ES Cells | Human iPS Cells | Mouse PS Cells | Human Fibroblasts |
hES-Cellect | √ | √ |
|
|
ES-Cellect | √ | √ | √ |
|
hFF-Cellect |
|
|
| √ |
產品應用
通過免疫細胞化學,流式細胞術和細胞磁珠分選鑒定和/或分離人ES和iPS細胞
從飼養(yǎng)細胞和分化后代分離人多能干細胞
重編程人類iPS細胞克隆的早期鑒定
染色表現(xiàn)
hES-Cellect
hiPSC on mEF, stained with hES-Cellect™ (1 µg/ml) and goat anti-mouse IgG Alexa Fluor 594 (2 µg/ml).
Staining performed according to protocol above. Pictures are shown in 20x magnification; scale bar corresponds to 50 µm. [a] Bright field, [b] Alexa Fluor 594, [c] DAPI.
ES-Cellect
hESC cultured on mEF, stained with ES-Cellect (1 µg/ml) and goat anti-mouse IgG Alexa Fluor 594 (2 µg/ml).
Staining performed according to the protocol above. Pictures are shown in 20x magnification; scale bar corresponds to 50 µm. [a] Bright field, [b] Alexa Fluor 594, [c] DAPI.
hFF-Cellect
hFF stained with hFF-Cellect™ (1 µg/ml) and goat anti-mouse IgG Alexa Fluor 594 (2 µg/ml).
Staining performed according to the protocol above. Pictures are shown in 20x magnification; scale bar corresponds to 50 µm. [a] Alexa Fluor 594, [b] DAPI, [c] overlapping.
訂購信息
品牌 | Code No. | 包裝量 | 價格 |
Cellartis | Y20010 | 150 μg | 5,954 元 |
Cellartis | Y20011 | 150 μg | 5,954 元 |
Cellartis | Y20012 | 150 μg | 5,954 元 |
華雅再生醫(yī)學旗艦公司:紅榮微再(上海)生物工程技術有限公司主營細胞治療和精準醫(yī)療等產品,2018年與TAKARA繼續(xù)合作,授權代理(上海)其旗下Cellartis干細胞研究制品和Rubicon單細胞文庫構建試劑。Cellartis公司位于瑞典,擁有iPS細胞等干細胞分化為肝細胞及臟器細胞的分化誘導技術和ES細胞,ips細胞,分化細胞等干細胞相關產品。
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